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Rev Diabet Stud, 2017, 14(1):22-38 DOI 10.1900/RDS.2017.14.22

Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?

Daniel Brandhorst, Heide Brandhorst, Paul R.V. Johnson

Nuffield Department of Surgical Sciences, University of Oxford, United Kingdom
Address correspondence to: Daniel Brandhorst, Nuffield Department of Surgical Sciences, University of Oxford, John Radcliffe Hospital, Level 6, Headley Way, OX3 9DU, Oxford, United Kingdom, email:

Manuscript submitted June 7, 2017; accepted June 9, 2017.

Keywords: diabetes, collagenase, islet isolation, neutral protease, thermolysin, tryptic-like activity, clostripain, extracellular matrix protein, basement membrane

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