Short Reports

Rev Diabet Stud, 2007, 4(4):226-230 DOI 10.1900/RDS.2007.4.226

Effectiveness of an Intensive Nutritional Intervention in Patients with Type 2 Diabetes Mellitus: Results from a Pilot Study

Mary Yannakoulia1, Kalliopi-Anna Poulia2, Eleni Mylona1, Meropi D. Kontogianni1,2

1Department of Nutrition and Dietetics Harokopio University, 70 El. Venizelou St, 17671, Athens, Greece
2Department of Nutrition and Dietetics, Laiko General University Hospital, 17 Ag. Thoma St, 11527, Athens, Greece
Address correspondence to: Mary Yannakoulia, e-mail: myiannak@hua.gr

Abstract

The aim of this pilot study was to compare the effects of an intensive nutritional intervention with usual care conditions on dropout rate, body weight, lifestyle changes and glycemic control in patients with type 2 diabetes mellitus (T2DM). Thirty outpatients with T2DM but without insulin treatment (mean age: 57 ± 9 yr) were randomly assigned to one of the two intervention groups: intensive care (IC) or usual care (UC). Patients in the UC group were given advice about dietary and physical activity goals in one consultation session at baseline, while patients in the IC group attended five goal-oriented consultation sessions held approximately every two weeks from baseline onwards. Changes in body weight, T2DM knowledge, dietary intake, physical activity, HbA1c, and percentage of dropouts were evaluated at 1-year follow-up post-intervention. Fifty percent of patients quitted the program and were classified as "dropouts". Program completers were older and included a lower percentage of newly diagnosed T2DM compared with dropouts. A tendency to a negative association between attendance of the IC group and the likelihood of dropping out was found (p = 0.08). No difference was detected between UC and IC groups regarding changes in body weight, HbA1c or other outcome measures, at post-intervention or 1-year follow-up. This pilot study did not confirm advantages of intensive nutritional intervention in T2DM patients in terms of glycemic control, body weight, diet and physical activity. However, the high dropout rate may have hampered its effectiveness.

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Rev Diabet Stud, 2007, 4(4):231-235 DOI 10.1900/RDS.2007.4.231

Evaluation of Apolipoprotein M Serum Concentration as a Biomarker of HNF-1alpha MODY

Jan Skupien1, Grzegorz Kepka1, Sylwia Gorczynska-Kosiorz2, Anna Gebska3, Tomasz Klupa1, Krzysztof Wanic1, Natalia Nowak1, Maciej Borowiec4, Jacek Sieradzki1, Maciej T. Malecki1

1Department and Chair of Metabolic Diseases, Jagiellonian University Medical College, Krakow, Poland
2Department of Internal Medicine, Diabetology and Nephrology, Medical University of Silesia, Zabrze, Poland
3Chair of Pharmacology, Jagiellonian University Medical College, Krakow, Poland
4Department of Pediatrics, Medical University of Lodz, Lodz, Poland
Address correspondence to: Maciej T. Malecki, e-mail: mmalecki@cm-uj.krakow.pl

Abstract

Apolipoprotein M (apoM) is a 26-kDa protein expressed mainly in the liver and kidneys. It is present predominantly in high-density lipoproteins (HDL). ApoM expression is influenced by the hepatocyte nuclear factor-1α (HNF-1α), which is a transcription factor associated with the pathogenesis of MODY. Some earlier data suggested that apoM levels were lower in the serum of HNF-1α MODY subjects, than in that of other diabetics and healthy controls. The aim of this study was to evaluate apoM as a biomarker for HNF-1α MODY. We included in this study 48 HNF-1α mutation carriers (40 diabetic patients and 8 subjects with normal glucose levels in the fasted state) from the Polish Nationwide Registry of MODY. In addition, we examined 55 T2DM patients and 55 apparently healthy volunteers who had normal fasting glucose levels. ApoM was measured by the sandwich dot-blot technique with recombinant apoM (Abnova) as a protein standard, mouse anti-human apoM monoclonal primary antibody and rat anti-mouse HRP-conjugated secondary antibody (BD Biosciences). Mean apoM level in the MODY group was 13.6 μg/ml, SD 1.9 (13.5 μg/ml, SD 1.7 in diabetic subjects and 13.9 μg/ml, SD 2.0 in non-diabetic mutation carriers respectively). In the T2DM group, mean apoM level was 13.7 μg/ml, SD 2.1, while it reached 13.8 μg/ml, SD 2.0 in healthy controls. There was no difference between apoM serum concentrations in all the study groups. In summary, our study showed no association between HNF-1α mutations resulting in MODY phenotype and apoM levels. Thus, we cannot confirm the clinical usefulness of apoM as a biomarker of HNF-1α MODY.

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